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KMID : 0380219830160010081
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Journal of Biochemistry and Molecular Biology
1983 Volume.16 No. 1 p.81 ~ p.86
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Biosynthesis of Bovine Pepsinogen
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Abstract
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Poly(A)-containing mRNA of pepsinogen was isolated from total RNA of bovine gastric mucosa by oligo(dT)-cellulose chromatography and Sepharose 4B chromatography, and was translated in a wheat germ cell-free system. The translation products were analyzed by ⁴sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. A major translation product was pepsinogen which was synthesized as two different molecular forms with molecular weights of 45,000 and 43,000 daltons.
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